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KMID : 0900920000240030255
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Korean journal of Animal Reproduction
2000 Volume.24 No. 3 p.255 ~ p.262
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Effect of Sperm Preincubation Medium with Ascorbic Acid and/or Ferrous Sulfate on Porcine In-Vitro Fertilization
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Park C.-K.
Nam H.-S.
Lee J.-H.
Kim I.-C.
Cheong H.-T.
Yang B.-K.
Kim C.-I.
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Abstract
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The aim of this work was to study the effects of ascorbic acid (Asc) and/or ferrous sulfate (Fe^{2+}) and spernatozoa preincubation on the in vitro fertilization in porcine. Porcine follicular oocytes matured in culture were inseminated with frozen-thawed boar spermatozoa preincubated for 0, 1, 2, 3, 4 and 5h. The penetration rates (37~51%) were not significantly different between durations of spermatozoa preincubation in medium with 0.1 mM Asc. The addition of 1.0 mM Fe^{2+} during spermatozoa preincubation were not significantly affecting the penetration rates (41~56%). When spermatozoa were preincubated with Asc and Fe^{2+}, the penetration rates had a tendency to increase with time of spermatozoa preincubation, and were significantly (P<0.05) higher in spermatozoa preincubated with that than without Asc and Fe^{2+} for 5 h. On the other hand, when spermatozoa were preincubated in fertilization medium without Asc and/or Fe^{2+}, the penetration rates were significantly (P<0.05) higher in medium with Fe^{2+} than with Asc or Asc and Fe^{2+} for in vitro fertilization. The rate of polyspermy in penetrated oocytes in medium with Asc and Fe^{2+} decreased with the period of spermatozoa preincubation. Despite different culture conditions for spermatozoa preincubation, no differences were observed in polyspermy rates in the presence of Asc and/or Fe^{2+} These results indicate the advantage of preincubating spermatozoa with Asc and Fe^{2+} and an addition of Fe^{2+} during in vitro fertilization with spermatozoa preincubated maintain penetration potential without increased polyspermy rates on in vitro fertilization in porcine oocytes.on in porcine oocytes.
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KEYWORD
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Ascorbic acid, Ferrous sulfate, in vitro penetration, Preincubation, Porcine
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